Coding

Part:BBa_K1923008

Designed by: Tianyang Mao   Group: iGEM16_Tsinghua   (2016-10-14)
Revision as of 21:39, 18 October 2016 by Maoty (Talk | contribs)


sgRNA targeting actin sense message (sgActin)

This part expresses small guide RNA targeting yeast Actin mRNA. With the assistance of PAMmer, a nucleotide oligo containing NGG sequence, it is able to help dCas9 to bind to the Actin mRNA transcripts, thus sequesters dCas9 in the cytoplasm. The transcription of this sgRNA is driven by tRNATyr promoter. HDV ribosome is a self-splicing element, which would process sgRNA to a mature transcript [1].

For more information, please refer to the Wiki page of Team Tsinghua.

Reference:
[1] Nelles D A, Fang M Y, O’Connell M R, et al. Programmable RNA tracking in live cells with CRISPR/Cas9[J]. Cell, 2016, 165(2): 488-496.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 122
    Illegal NgoMIV site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None