RNA

Part:BBa_K1941002

Designed by: Dimitri Coukos   Group: iGEM16_EPFL   (2016-10-13)
Revision as of 21:38, 18 October 2016 by Alixfailletaz (Talk | contribs) (→‎Usage and Biology)

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scTef1_2PP7

Scaffold RNA (scRNA), with two times the PP7 hairpin, targeting TEF1 promoter.


Usage and Biology

A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding module. In this case, the hairpins are two times PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter TEF1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None