Device

Part:BBa_K2040121

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-14)
Revision as of 20:54, 18 October 2016 by Chilam Poon (Talk | contribs)


PgpdA + KillerRed + TtrpC

A KillerRed expression cassette was created with a fungal promoter PgpdA and a fungal terminator TtrpC.


Usage and Biology

KillerRed,always used for precise inactivation of selected proteins in chromophore-assisted light inactivation (CALI) technique and for the light-induced cell killing, is rarely used to express in fungal chassis.

NYMU 2016 construct this new device, for fungal expression, to test wheather KillerRed protein can be used in entomopathogenic fungi Metarhizium anisopliae, our chassis organism.

We demonstrated the functionality of this part via transforming this expression cassette into M.anisopliae. The transformants were under a fluorescence microscope(Magnification: 400×):


  • Wildtype:
WT FL.png
  • KillerRed transformant:
KR FL.png


KillerRed proteins were successfully expressed in M.anisopliae.









Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 880
    Illegal BsaI.rc site found at 1171


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Categories
Parameters
None