Part:BBa_K2008008

pVeg->sec-TD1 modular secretion platform with ThrC homology

This part is meant to be used as a way to integrate any desired insert into the B. subtilis chromosome, which will then be secreted and possess the ability to diffuse transdermally through human skin.

The integration cassette contains the constitutive B. subtilis promoter pVeg (https://parts.igem.org/Part:BBa_K143012) and a strong B. subtilis RBS (https://parts.igem.org/Part:BBa_K780001), as well as a B. subtilis secretory signal peptide sequence to allow for secretion of the desired protein product directly into surrounding media. A transdermal tag, TD1, (https://parts.igem.org/Part:BBa_K1074000) is included to allow for diffusion of the protein product across the skin.

The sequence contains two internal restriction enzyme sites: one for BamHI and one for XmaI. These sites allow for the directional cloning of a desired insert.

Note: This part is a composite part, not a basic part, but it could not be added as composite at the time of entry.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 313
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]