Device

Part:BBa_K2008004

Designed by: Rachelle Varga   Group: iGEM16_UofC_Calgary   (2016-10-08)
Revision as of 19:21, 18 October 2016 by Rcvarga (Talk | contribs)

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pVeg->sec-TD1-BBI

This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI). A B. subtilis secretory signal peptide sequence is included to allow for secretion of the peptide directly into surrounding media. A transdermal tag, TD1 (https://parts.igem.org/Part:BBa_K1074000) is fused to the N-terminus of BBI to allow for diffusion of the peptide across the skin.

This coding sequence is under the control of the constitutive B. subtilis promoter pVeg (https://parts.igem.org/Part:BBa_K143012) and a strong B. subtilis RBS (https://parts.igem.org/Part:BBa_K780001).

Note: This part is a composite part, not a basic part, but it could not be added as composite at the time of entry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None