Composite

Part:BBa_K2040119:Design

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-12)
Revision as of 18:44, 18 October 2016 by Chilam Poon (Talk | contribs) (→‎Design Notes)

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mRFP1 + TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1351
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1065


Design Notes

Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.

Source

mRFP1 orignated from Discosoma striata; TtrpC was isolated from pAN7-1.

References