Terminator

Part:BBa_K2040102:Design

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-11)
Revision as of 18:01, 18 October 2016 by Chilam Poon (Talk | contribs) (→‎Design Notes)


Fungal terminator TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 351


Design Notes

One illegal cutsite (XbaI) were subsequently removed via site-directed mutagenesis.

Source

It was taken from vector pAN7-1.

References