Regulatory

Part:BBa_K2040101:Design

Designed by: Chilam Poon   Group: iGEM16_NYMU-Taipei   (2016-10-11)
Revision as of 17:56, 18 October 2016 by Chilam Poon (Talk | contribs) (→‎Design Notes)


Fungal promoter PgpdA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 903
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 137
    Illegal XhoI site found at 693
    Illegal XhoI site found at 1075
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
    Illegal AgeI site found at 1430
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109
    Illegal SapI site found at 315


Design Notes

One illegal cutsites (PstI) were subsequently removed via site-directed mutagenesis.

Source

It was taken from vector pAN7-1.

References