Coding

Part:BBa_K1965001

Designed by: Nik Franko   Group: iGEM16_Slovenia   (2016-10-17)
Revision as of 16:06, 18 October 2016 by NinaJerala (Talk | contribs)


TRPC1:Myc

Transient receptor potential channel 1 (TRPC1) is a human non-specific cation channel located at the plasma membrane. It has been previously reported as broadly expressed in human tissues where it functions as a store-operating calcium channel [1]. It belongs to the TRP superfamily that act as tetrameric transmembrane proteins, consisting of a domain formed by six transmembrane helices, with a pore between S5 and S6 [2]. These helices present the N- and C-termini to the cytoplasm, promoting the formation of functional homo- or hetero-tetramers [3]. The construct contains a Myc tag on the C-terminus, facing the cytoplasm (1A).

Localization and expression of mechanosensitive ion channels MscS and TRPC1.
(A) Scheme of bacterial ion channel MscS (top) and human ion channel TRPC1 (lbottom). (B) Ion channel MscS is localized on the plasma membrane. (C) TRPC1 is predominantly localized in the ER. (D) Ion channels MscS and TRPC1 are expressed in HEK293 cells. HEK293 cells were transfected with plasmids encoding HA tagged MscS or Myc-tagged TRPC1. Expression by Western blot and localization by confocal microscopy were analyzed using anti-HA and anti-Myc antibodies, respectively.

References

[1]Xu, S. & Beech, D. J. TrpC1 Is a Membrane-Spanning Subunit of Store-Operated Ca2+ Channels in Native Vascular Smooth Muscle Cells. Circ. Res. 88, 84–87 (2001).
[2]Nilius, B., Owsianik, G., Voets, T. & Peters, J. a J. a. Transient receptor potential cation channels in disease. Physiological … 87, (2007).
[3]Bianchi, L. Mechanotransduction: Touch and Feel at the Molecular Level as Modeled in Caenorhabditis elegans. Mol. Neurobiol. 36, 254–271 (2007).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 655
    Illegal BglII site found at 3099
    Illegal BamHI site found at 649
    Illegal BamHI site found at 850
    Illegal BamHI site found at 1494
    Illegal XhoI site found at 2789
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 73
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None