Composite

Part:BBa_K1898550:Design

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 15:31, 18 October 2016 by Fionat17112752 (Talk | contribs)

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Strong promoter + Strong RBS + GFP + 10x Histidine tag + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

When we designed the GFP construct, we took out the double stop codon at the end of BBa_E0040.

The following primers were used to move the GFP construct from IDT into iGEM BioBrick:

Forward: 5' ATATgAATTCgCggCCg 3' (17)

Reverse: 5' ATATCTgCAgCggCC 3' (15)


Source

This construct was designed and sent to IDT for oligo synthesis. Primers were synthesized by Tri-I BioTech