Composite

Part:BBa_K1897024

Designed by: Keshiniy Madivannan   Group: iGEM16_NUS_Singapore   (2016-10-11)
Revision as of 13:37, 18 October 2016 by Phuongnguyen (Talk | contribs)


p62-33-ALR-Terminator

The construct contains a shorter version of the promoter region for wild-type lldPRD operon (Part:BBa_K1897037) derived from (Part:BBa_K822000) with a weaker RBS flanked by non-coding sequences (Part:BBa_K1897031), followed by a coding sequence of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 499
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 423
    Illegal BamHI site found at 1125
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 541
    Illegal AgeI site found at 841
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 298


[edit]
Categories
Parameters
None