Part:BBa_K1993030

CXCR5-T2A-Luciferase-IRES-eGFP

Functions:

With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we constructed a plasmid BBa_K1993030 under the control of EF-1α.   

Details:

• Elongation factor-1α (EF-1α), as a constitutive promoter, is responsible for mediating the recruitment of aminoacyl-tRNA to the A-site of the 80S ribosome during protein synthesis.
• CXCR5 is a chemokine receptor and its corresponding ligand is CXCL13. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993013 BBa_K1993013)
• T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019)
• Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is distinct from a photoprotein. (Details could be seen on BBa_K1993015)
• Internal ribosome entry site (IRES) is an RNA element that allows for translation initiation in an end-independent manner, as part of the greater process of protein synthesis. (Details could be seen on BBa_K1993016)
• eGFP dramatically improved the spectral characteristics of GFP and allowed the practical use of GFP in mammalian cells. (Details could be seen on BBa_K1993017) Sequence and Features

  Assembly Compatibility:

  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]

    Illegal BglII site found at 1398
    Illegal BamHI site found at 1941
    Illegal XhoI site found at 31
    
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]

    Illegal NgoMIV site found at 973
    
  • 1000
    INCOMPATIBLE WITH RFC[1000]

    Illegal BsaI.rc site found at 259
    Illegal SapI site found at 1050