Composite

Part:BBa_K1918106

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 12:20, 18 October 2016 by SheepW (Talk | contribs)


CMV-Kozak-EBFP2N(1-154):IntN

This part is consist of EBFP2N(1-154):IntN and the CMV promoter. EBFP2N(1-154):IntN is a peptied made up of the 1st(N-terminus) to 154th amino acids in fluorescent protein EBFP2 and IntN. Driven by CMV promoter, the EBFP2N(1-154):IntN could be expressed all the time.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 755
    Illegal SpeI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 755
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1499
    Illegal SpeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


[edit]
Categories
Parameters
None