Composite

Part:BBa_K1918105

Designed by: Linghan Wang   Group: iGEM16_Tsinghua-A   (2016-10-14)
Revision as of 12:17, 18 October 2016 by SheepW (Talk | contribs)


CMV-Kozak-EBFP2C(155-238)

This part is consist of EBFP2C(155-238) and the CMV promoter. EBFP2C(155-238) is a peptied made up of the 155th to 238th(C-terminus) amino acids in fluorescent protein EBFP2. Driven by CMV promoter, the EBFP2C(155-238) could be expressed all the time.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1025
    Illegal SpeI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 755
    Illegal SpeI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 755
    Illegal BamHI site found at 738
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1025
    Illegal SpeI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 755
    Illegal XbaI site found at 1025
    Illegal SpeI site found at 83
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 716


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Categories
Parameters
None