Coding

Part:BBa_K1898100:Design

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 11:48, 18 October 2016 by Fionat17112752 (Talk | contribs)

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ch25h, cholesterol 25 hydroxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CH25H was ordered with one base pair changed to remove an internal cutting site and the stop codon was removed as well.

PCR primers were designed to clone CH25H into a Biobrick backbone

forward: 5' ATATgAATTCgCggCCg 3' (17)

reverse: 5' ATATCTgCAgCggCC 3' (15)


Source

CH25H was synthesized by IDT and the primers were synthesized by Tri-I Biotech.