Intermediate

Part:BBa_K2088009

Designed by: Xia Chen   Group: iGEM16_NAU-CHINA   (2016-10-08)
Revision as of 10:36, 18 October 2016 by Summerice (Talk | contribs)


nativeRBS-C23O with double Terminator

This part includes catechol 2,3-dioxygenase(C23O) gene from Sphingobium sp. YBL2 with native RBS and a double Terminator(B0015). This part functions as both a reporter and a degradation gene, since C23O converts catechol to 2-hydroxymuconate semialdehyde, which shows a bright yellow color. When ligated with another gene or an unknown promoter, this part can be used to test the expression of gene and the strength of promoter just utilizing catechol solution as "chromogenic reagent". 

I

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 839


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Categories
Parameters
None