Composite

Part:BBa_K1898550

Designed by: Fiona Tsai   Group: iGEM16_TAS_Taipei   (2016-10-10)
Revision as of 10:30, 18 October 2016 by Fionat17112752 (Talk | contribs)


Strong promoter + Strong RBS + GFP + 10x Histidine tag + double terminator

Strong promoter and strong rbs (BBa_K880005) is used to maximize protein output. The ORF includes green fluorescence protein and a 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.

This part can be used to test protein purification method or be used as a way to quantify protein expression level.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


synthesis report

This construct is synthesized by IDT. The following sequence was sent to IDT to synthesize, and each feature is annotated as shown below:

Bpht_sequence.png

IDT report shows that the sequence is successfully synthesized:

Bpht_idt_2.png

Bpht_idt_1.png

[edit]
Categories
//awards/part_collection/2016
Parameters
None