Composite

Part:BBa_K1997013:Design

Designed by: Xinyuan Qiu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 08:43, 18 October 2016 by Zhuchushu13 (Talk | contribs) (→‎Design Notes)

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P+R->sHRP-N->Zif-N->RBS->Zif-C->sHRP-C->TER


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 382
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 624
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1003
    Illegal AgeI site found at 1087
    Illegal AgeI site found at 1207
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1210
    Illegal BsaI.rc site found at 907
    Illegal SapI.rc site found at 1324


Design Notes

Two restriction sites for Gibson assembly was added at the flank of "Zif-N-RBS-Zif-C" sequence, so that such sequence could be sassily replaced.

Source

This part was synthesized by company

References