Coding

Part:BBa_K2074023

Designed by: Yi Cai   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 08:33, 18 October 2016 by Heao (Talk | contribs)


Cry11Aa(Codon optimization)-Extended FMDV 800px-T--FAFU-CHINA--partsclones.png
our part is cry11Aa which have toxicity to the larve of mosquit link with 2A peptide.We transferred the gene cry11Aa+2A into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 1681
    Illegal NgoMIV site found at 2029
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49
    Illegal BsaI.rc site found at 244
    Illegal BsaI.rc site found at 1459
    Illegal SapI.rc site found at 1440


[edit]
Categories
Parameters
None