Coding

Part:BBa_K2074022

Designed by: Yi Cai   Group: iGEM16_FAFU-CHINA   (2016-10-09)
Revision as of 08:33, 18 October 2016 by Heao (Talk | contribs)


Cry10Aa(Codon optimization)-Extended FMDV 800px-T--FAFU-CHINA--partsclones.png
our part is cry10Aa which have toxicity to the larve of mosquit link with 2A peptide.We transferred the gene cry10Aa+2A into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 368
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 32
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal NgoMIV site found at 223
    Illegal NgoMIV site found at 280
    Illegal NgoMIV site found at 784
    Illegal NgoMIV site found at 1460
    Illegal NgoMIV site found at 2116
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 202
    Illegal SapI.rc site found at 924


[edit]
Categories
Parameters
None