Generator

Part:BBa_K1979003

Designed by: MA Xinyi   Group: iGEM16_SDSZ_China   (2016-08-26)
Revision as of 03:20, 18 October 2016 by Xinyiloowhoo (Talk | contribs)

PBP5 coding device (promoter+PBP5+T7 terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 135
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1178
    Illegal BamHI site found at 168
    Illegal XhoI site found at 1386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 492
    Illegal AgeI site found at 1094
  • 1000
    COMPATIBLE WITH RFC[1000]

Figure1. Double enzyme digestion check for BBa_K1979003. PBP5 has an expected size of 1200 bps (red box).

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