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Part:BBa_K2066550

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)
Revision as of 01:40, 18 October 2016 by Ethan801 (Talk | contribs)


85x tetO Binding Array

Part containing 85 tetO (tetR binding) sites. This part is used to induce a rightward shift in a transfer function by way of molecular titration.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2434
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2434
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1593
    Illegal BamHI site found at 825
    Illegal BamHI site found at 959
    Illegal BamHI site found at 1109
    Illegal BamHI site found at 1726
    Illegal BamHI site found at 1934
    Illegal BamHI site found at 2515
    Illegal BamHI site found at 3081
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2434
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2434
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2893
    Illegal SapI.rc site found at 160
    Illegal SapI.rc site found at 2506


[edit]
Categories
Parameters
None