Composite

Part:BBa_K1899006

Designed by: iGEM2016_HKUST   Group: iGEM16_Hong_Kong_HKUST   (2016-10-10)
Revision as of 23:45, 17 October 2016 by Leona (Talk | contribs)


J23101-B0032-lacl-B1006- phlFp-GFP

The construct aims at investigating any interference caused by lacl repressor on promoter PhlFp.

Results

748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg

Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and pSB3K3-PhlFp - BBa_E0240

The fold change between pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240) is 13.2, which is same as that of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240.Mean and standard error mean(SEM) are calculated and represented by the height of the bar and the error bar respectively. 

Since their RFU are similar and almost the same, it proves that there is no cross-talk between lacp and Phlfp(BBa_K1899004).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2007


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None