Composite

Part:BBa_K1899006

Designed by: iGEM2016_HKUST   Group: iGEM16_Hong_Kong_HKUST   (2016-10-10)
Revision as of 23:44, 17 October 2016 by Leona (Talk | contribs)


J23101-B0032-lacl-B1006- phlFp-GFP

The construct aims at investigating any interference caused by lacl repressor on promoter PhlFp.

Results

748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg

Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240 and pSB3K3-PhlFp - BBa_E0240

The fold change between pSB3K3-PhlFp - BBa_E0240 and negative control (pSB3K3-BBa_E0240) is 13.2, which is same as that of pSB3K3-BBa_J23101-B0032-C0012-B1006- PhlFp -E0240. Since their RFU are similar and almost the same, it proves that there is no cross-talk between lacp and Phlfp(BBa_K1899004).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2007


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
None