Protein_Domain

Part:BBa_K2136002

Designed by: Livia Seno Ferreira Camargo   Group: iGEM16_USP_UNIFESP-Brazil   (2016-10-13)
Revision as of 22:39, 17 October 2016 by Bcamposalazar (Talk | contribs)


Lysostaphin

Lysostaphin is a zinc metalloproteinase (EC 3.4.24.75) produced by Staphylococcus simulans. This enzyme hydrolyses Glycine/Glycine bonds in the pentaglycine interpeptide that maintain the stability of peptidoglycans in staphylococcal cell wall [1]. Lysostaphin rapidly lyses actively growing and non-dividing cells including staphylococci in biofilms and due to its specificity, it could have high potential in the treatment of antibiotic-resistant staphylococcal infections [2]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 505
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

PCR from pSB1C3 + Lysostaphin


T--USP_UNIFESP-Brazil--Lysosfromplasmid.png">


[edit]
Categories
Parameters
None