Coding

Part:BBa_K1965008

Designed by: Nik Franko   Group: iGEM16_Slovenia   (2016-10-17)
Revision as of 22:21, 17 October 2016 by Registry (Talk | contribs)

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His:CIBN:cLuc:HA

This part is one of the two components of the dimerazing system which is induced by blue light. We adapted this system for the reconstitution of split luciferase to create a blue-light sensor. To the C-terminus of CIBN, C-terminus of split firefly luciferase was added. Upon induction with light and concequent dimerisation, the split luciferase is reconstituted and regains activity, which results in biolumiescence if luciferin is added.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 130
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None