Part:BBa_K1886006
broken Ptet-cph8
The red light sensor (Cph8) is a fusion protein which is consisted of a phytochrome Cph1 and a histidine kinase domain, Envz-OmpR, that includes a response regulator.Cph1 is the first member of the plant photoreceptor family that has been identified in bacteria. The functional expression of a phytochrome domain(Cph1) in E. coli requires the biosynthesis of the respective bilin chromophore PCB. EnvZ-OmpR, a dimeric osmosensor, is a multidomain transmembrane protein and one of the best characterized two-component histidine kinases from E.coli.
With the biosynthesis of PCB, Cph1 serve as a red-light absorbing chromophore that is inactivated under red light and activated without red light. Upon changes of extracellular osmolarity, EnvZ specifically phosphorylates its cognate response regulator OmpR, which, in turn, regulates the PompC.
This circuit is composed of the constitutive promoter -- broken Ptet and membrane protein cph8. Under dark conditions, protein cph8 could transfer its phosphoryl group to intracellular protein ompR (ompR is native protein carried by E.coli), and this process could be inhibited by 650-nm light.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2332
Illegal XhoI site found at 439 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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