Coding
TetX

Part:BBa_K2150101:Design

Designed by: Zou Zhuoning   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 17:08, 17 October 2016 by Zepeng Mu (Talk | contribs) (→‎References)


Tetracycline resistance protein from Bacteroides fragilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 562
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

protein sequence--gene sequence(codon preferance) 3 synonynous mutaions

Source

TetX gene is initially found in the transposons Tn4351 and Tn4400 in the anaerobe Bacteroides fragilis We found the sequences of this tetracycline resistance protein in UniProt Protein Database. According to the codon bias in Escherichia coli, we transform the protein sequence into the gene sequence and had it commercially synthesized. In order to avoid illegal restriction sites, we conduct three synonymous mutations in our sequence.