Reporter

Part:BBa_K2150015

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)
Revision as of 15:52, 17 October 2016 by Forevyoung (Talk | contribs)


pTet TetX-GFP(fusion protein)

This part is the fusion protein of tetX-GFP with pTet promoter. PTet promoter is constitutively on and can be repressed by TetR, and the repression can be inhibited by tetracycline and its analogs. This part shows a lower fluoresence intensity than BBa_2150014 at the same growth stage of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1939


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Categories
Parameters
None