Device

Part:BBa_K2074003:Design

Designed by: Tong Tong   Group: iGEM16_FAFU-CHINA   (2016-10-17)
Revision as of 15:28, 17 October 2016 by T Square (Talk | contribs) (→‎Source)

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Cry4Aa+2A+Cyt1 expressed in C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 251
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1641
    Illegal BglII site found at 4452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2033
    Illegal NgoMIV site found at 4286
    Illegal NgoMIV site found at 4586
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 686
    Illegal BsaI.rc site found at 839
    Illegal BsaI.rc site found at 2726
    Illegal BsaI.rc site found at 3266
    Illegal BsaI.rc site found at 3575
    Illegal BsaI.rc site found at 4142
    Illegal SapI.rc site found at 1843
    Illegal SapI.rc site found at 1885


Design Notes

none


Source

HSP70A-RBCS2 promoter cloned from Chlamydomonas reinhardtii. Cry4Aa and Cyt1 cloned from Bacillus thuringiensis serovar israelensis strain BRC-LLP29, codon optimal for Chlamydomonas reinhardtii. Extended FMDV 2A peptides cloned from FMDV, codon optimal for Chlamydomonas reinhardtii.

References