Coding

Part:BBa_K1985012

Designed by: Hanya Benotmane   Group: iGEM16_Kent   (2016-10-14)
Revision as of 15:27, 17 October 2016 by JJECornish (Talk | contribs) (→‎Usage and Biology)

[CsgA-SS-Sup35NM]

This part is an improved version of a previously designed BioBrick (Part:BBa_K1739002), which was designed by the Kent 2015 iGEM team. The promoter BBa_J23104 has been removed. The part contains two segments in one biobrick: CsgA signal sequence and Sup35NM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 671


Usage and Biology

The part was used in pSB1A3.

For more information on biology and usage see part BBa_K1739002

Validation

The part was first validated with a diagnostic restriction digest using EcoRI and PstI and agarose gel electrophoresis. The expected band sizes from the digest were: xxxx for the plasmid backbone and xxx for the insert. A 1kB plus DNA marker was used to verify the sizes of the bands and it was confirmed that the correct plasmid had been produced.

File:Filename.jpeg
Figure 1. Agarose gel of the restriction digest of BBa_K1985012 in pSB1C3, with EcoRI and PstI.


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