Composite
Part:BBa_K2060000:Design
Designed by: Cardiff_Wales Team, Geraint Parry Group: iGEM16_Cardiff_Wales (2016-09-23)
CRISPR-Cas9 guide RNA targeting to 16S RNA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 48
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
One of the Cardiff_Wales iGEM instructors Daniel Pass, designed a bioinformatic tool for the selection of appropriate guide RNAs that adhered to the following rules:
Used standard design considerations for guide RNAs
Source
E.coli 16S ribosomal RNA