Composite

Part:BBa_K2060000:Design

Designed by: Cardiff_Wales Team, Geraint Parry   Group: iGEM16_Cardiff_Wales   (2016-09-23)
Revision as of 14:44, 17 October 2016 by Gparry75 (Talk | contribs)


CRISPR-Cas9 guide RNA targeting to 16S RNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

One of the Cardiff_Wales iGEM instructors Daniel Pass, designed a bioinformatic tool for the selection of appropriate guide RNAs that adhered to the following rules:



Used standard design considerations for guide RNAs


Source

E.coli 16S ribosomal RNA

References