Coding

Part:BBa_K2020050:Design

Designed by: Andrea Hoeltken, Carolina Bonerath, Vroni Czotscher   Group: iGEM16_Aachen   (2016-10-02)
Revision as of 13:20, 17 October 2016 by Andrea hoeltken (Talk | contribs) (→‎References)

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wild type Mj Y-Synthetase for use in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 84
    Illegal SapI.rc site found at 877


Design Notes

Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a low copy plasmid (e.g. pACYC) which fits your needs.

[http://2016.igem.org/Team:Aachen Team Aachen 2016] used synthetases in pACYC with p15A-ORI and Gent-Resistance. Together with a tRNA (BBa_K2020042) with own Ipp-Promoter and Terminator.



Source

wild type tyrosyl Methanococcus janaschii tRNA-Synthetase. Obtained via iGEM Team 2014 Austin, Texas.

References

  1. Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion