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Plasmid

Part:BBa_K1930003:Design

Designed by: Barbora Waclawikova   Group: iGEM16_Groningen   (2016-10-16)
Revision as of 10:56, 17 October 2016 by Barawaclawik (Talk | contribs)

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Encrypted message sequence in integration plasmid (BBa_K823023)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6601
    Illegal XhoI site found at 4109
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1663
    Illegal NgoMIV site found at 4073
    Illegal NgoMIV site found at 5212
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5036


Design Notes

The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The message sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted message sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our message sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments


Source

Message sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding

BBa_K823023: Created by iGEM Munich 2012. https://parts.igem.org/Part:BBa_K823023

References

[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]