Composite

Part:BBa_K1949101

Designed by: Yoshio Takata   Group: iGEM16_Tokyo_Tech   (2016-10-11)
Revision as of 02:12, 17 October 2016 by Kazuki (Talk | contribs) (→‎result)


PBAD-rbs-mazF

Characterize

We performed experiment to work final genetic circuits.

This experiment consists of the four parts below.

Ⅰ.Adjustment of MazF Expression

Ⅱ.

Ⅲ.

Ⅳ.

Adjustment of MazF Expression

In order to control the TA system well, it is necessary to adjust the expression of MazF as a toxin. It has been reported that MazF has very strong ability to inhibit cell growth, and antitoxins cannot recover it under overexpression of MazF. Therefore, we explored the conditions that can control the TA system by adjusting the expression of MazF.

result

It was found that the cell growth is inhibited when the concentration of arabinose, the inducer of MazF, is more than 0.02%. However, when arabinose concentration is 0.2%, GFP fluorescence intensity falls markedly.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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