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Plasmid

Part:BBa_K1930002:Design

Designed by: Eike Mahlandt   Group: iGEM16_Groningen   (2016-10-16)
Revision as of 23:45, 16 October 2016 by Barawaclawik (Talk | contribs) (→‎Source)


Encryption key sequence in integration plasmid (BBa_K823023)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 6045
    Illegal XhoI site found at 3553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1107
    Illegal NgoMIV site found at 3517
    Illegal NgoMIV site found at 4656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4480


Design Notes

The iGEM team Groningen 2016 worked on bioencryption in order to safely store data in DNA. In our project we were mainly working with two sequences of DNA which were the encrypted message sequence and the encryption key sequence. The key sequence was created by our software. It is therefore artificial DNA. We ordered the encrypted key sequence as gBlock from IDT (Integrated DNA technologies). This part was created in the interest of having a genomic integration plasmid with our key sequence. We used an available BioBrick BBa_K823023 from igem Munich 2012. It is an integration plasmid for Bacillus subtilis, which can be used for cloning in E. coli as well. An RFP is inserted in BBa_K823023 for more efficient screening after transformation. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments


Source

Key sequence: Created by our CryptoGErM software. http://2016.igem.org/Team:Groningen/Encoding

BBa_K823023: Created by iGEM Munich 2012. https://parts.igem.org/Part:BBa_K823023

References

[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]