Protein_Domain

Part:BBa_K2136018

Designed by: Tiago Lubiana Alves   Group: iGEM16_USP_UNIFESP-Brazil   (2016-10-16)
Revision as of 23:42, 16 October 2016 by Lubdub (Talk | contribs)

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Ars2 signal peptide for secretion in Chlamydomonas

This sequence is part of the arylsuflatase 2 (Ars2) gene in Chlamydomonas reinhardtii. It is responsible for allowing the secretion of the fused protein to the culture medium, thus allowing cell-lysys independent purification of proteins and/or secretion for other aplications

This part also includes a 3' scar with XhoI and ClaI restriction sites included in original gene synthesis to better suit team USP_UNIFESP 2016's workflow.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None