Regulatory
PAtpI

Part:BBa_K1930005:Design

Designed by: Eike Mahlandt   Group: iGEM16_Groningen   (2016-10-16)
Revision as of 22:32, 16 October 2016 by Barawaclawik (Talk | contribs) (→‎References)


PAtpI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter PAtpI is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.

The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams. For more information see plasmid construction under our lab journal: http://2016.igem.org/Team:Groningen/Experiments


Source

Ordered as gBlock. IDT (Integrated DNA technologies).

References

Sinai et al.

[http://subtiwiki.uni-goettingen.de/bank/index.php?gene=atpA Subtiwiki-atpA]