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Regulatory
PAtpI
Part:BBa_K1930005
Designed by: Eike Mahlandt Group: iGEM16_Groningen (2016-10-16)
Revision as of 22:18, 16 October 2016 by Barawaclawik (Talk | contribs)
PAtpI
The promoter PAtpI has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 min of germination Sinai et al.. This gene is part of an operon, therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.
The region was checked for the binding of sigma factors and transcription factors with DBTBS. No binding factors were found with the highest significance level. In our project we wanted to find a constitutive ON promoter for our ciprofloxacin resistance casette. In the following part we put the promoter PAtpI in the pSB1C3 backbone to make it available to other iGEM teams.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/prokaryote/bsubtilis
//function/regulation/transcriptional
//regulation/positive
//function/regulation/transcriptional
//regulation/positive
control | |
positive_regulators |