Coding

Part:BBa_K1962001

Designed by: Frank Sargent   Group: iGEM16_Dundee   (2016-10-11)
Revision as of 20:50, 16 October 2016 by Frulhuq (Talk | contribs)


Immunity Protein Im-Ia

This biobrick codes for the Immunity Protein for Colicin Ia (BBa_K1962000). Colicin Ia is a channel-forming bacteriocin that depolarizes the cytoplasmic inner membrane of target bacteria, leading to dissipation of cellular energy. This Immunity Protein is tightly linked to its specific Colicin bacteriocin domain to protect the colicinogenic cell from the cytotoxic activity of the colicin.

Usage and Biology

Cloning Strategy

Our aim was to create a device which would express Colicin Ia in response to pH and bile salts. We cloned the Ia-Immunity protein downstream of a pH sensitive promoter Pasr (BBa_K1231000) and a bile salt sensitive promoter PacrRA (BBa_K1231001) to generate the composite parts (BBa_K1962015) and (BBa_K1962011), respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
    Illegal AgeI site found at 253
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None