Reporter
GG100

Part:BBa_K2145107

Designed by: Laura Xinyu Chen, Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-14)
Revision as of 20:05, 16 October 2016 by Mwu (Talk | contribs)


GFP/RFP reporter (Reverse/Forward) with random 500bp spacer

This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 825
    Illegal AgeI site found at 937
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None