Coding
Csp1

Part:BBa_K1980000

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 19:57, 16 October 2016 by Sgarforth (Talk | contribs)


TAT Copper Storage Protein 1

Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and added a C terminal his tag to aid purification.

Sequence and Features:


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//chassis/prokaryote/ecoli
//collections/probiotics/production
Parameters
None