Composite

Part:BBa_K2066040:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)
Revision as of 17:47, 16 October 2016 by Jlmaniaci (Talk | contribs) (→‎Source)


J23100 + RiboJ Promoter Characterization Part with 2x Broccoli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

to be completed


Source

The UNS2, UNS3, and UNS6 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). The spacer sB is taken from Pothoulakis et al. 2013 (“The Spinach RNA Aptamer as a Characterization Tool for Synthetic Biology”). The broccoli sequence is from https://www.addgene.org/66843/. The mCherry sequence is taken from WM16_029 with the start codon and extra stop codon added.

References