Composite

Part:BBa_K2066040

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)
Revision as of 17:47, 16 October 2016 by Jlmaniaci (Talk | contribs)


J23100 + RiboJ Promoter Characterization Part with 2x Broccoli

This part is meant for use in Promoter Characterization and RBS Characterization subprojects for WM iGEM 2016.

This part allows for simultaneous characterization of gene expression at the RNA and Protein level using the broccoli aptamer and mCherry fluorophore respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 47
    Illegal NheI site found at 70
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None