Measurement

Part:BBa_K2066037

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-08)
Revision as of 17:00, 16 October 2016 by Jlmaniaci (Talk | contribs)


pACT-Tet on UNS Standard

This is the pACT-Tet sequence from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper flanked by UNS 2/3. This is meant for use in Tet based synthetic enhancer systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 969
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None