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Part:BBa_K1943020:Design

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-10-13)
Revision as of 14:00, 16 October 2016 by John Tsq (Talk | contribs) (→‎Design Notes)


R-GECO


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 914
    Illegal SapI.rc site found at 111
    Illegal SapI.rc site found at 820


Design Notes

This TetOn expression system contains a SV40 promoter, a puro anti-antibiotics gene, 2A and TetOn 3G promoter. We design primers and do PCR of this TetOn expression system and ligate it to pSB1C3 backbone.

Source

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References