Reporter

Part:BBa_K1943010:Design

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-09-04)
Revision as of 13:48, 16 October 2016 by John Tsq (Talk | contribs) (→‎Design Notes)


msfGFP, green fluorescence protein reporter system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 74


Design Notes

In our lab, we usually use sfGFP as a report for molecular experiment. In the plasmid, there is a sfGFP coding device which contains promoter BBa_J23116 and RBS BBa_B0034. Some of our backbones contain KpnI site, and we need a single KpnI site, while sfGFP contains a KpnI site. So we do site-directed mutagenesis of it. And then we design primer and do PCR of this msfGFP coding device and ligate it to pSB1C3 backbone.

Source

msfGFP

References