Reporter

Part:BBa_K1943002:Design

Designed by: Shixin Lu, Shiqiang Tang   Group: iGEM16_SUSTech_Shenzhen   (2016-08-29)
Revision as of 13:15, 16 October 2016 by John Tsq (Talk | contribs) (→‎Source)


gfasPurple, purple chromoprotein reporter system (Strong Promoter, Strong RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no


Source

The following list is the Biobricks we’ve used in construction and their detailed design information.

Part Type Discription Backbone Location on the plate
BBa_K880005 Promoter + RBS Strong Promoter + Strong RBS pSB1C3 3F 2016 Kit Plate 2
BBa_K608007 Promoter + RBS Medium Promoter + Weak RBS pSB1C3 5G 2016 Kit Plate 1
BBa_K592012 Chromoprotein eforRed, red chromoprotein pSB1C3 15I 2016 Kit Plate 6
BBa_K1033919 Chromoprotein gfasPurple, purplr chromoprotein pSB1C3 9K 2016 Kit Plate 6
BBa_K1033910 Chromoprotein fwYellow, yellow chromoprotein pSB1C3 6K 2016 Kit Plate 4
BBa_K1033932 Chromoprotein spisPink, pink chromoprotein pSB1C3 11K 2016 Kit Plate 6
BBa_B0015 Terminator Double terminator pSB1C3 3F 2016 Kit Plate 3

References