Coding

Part:BBa_K1985003

Designed by: Jasmine Cornish   Group: iGEM16_Kent   (2016-10-14)
Revision as of 12:22, 16 October 2016 by JJECornish (Talk | contribs)

mamP, his-tagged, signal sequence cleaved

This part is an differentiation on Part:BBa_K1985000.

Usage and Biology

This part has a his-tag included so was used to purify the expressed mamP protein. MamP is usually targeted to the membrane, however in this part the targeting sequence has been cleaved and it should instead be targeted to the periplasm.

It was expressed, purified and then exposed to iron.

For more information on its biology and usage, see part BBa_K1985000.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 517
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None