Generator

Part:BBa_K2012013

Designed by: Wenqi Huang   Group: iGEM16_HZAU-China   (2016-10-06)
Revision as of 11:50, 16 October 2016 by Johnson Huang (Talk | contribs)


Strong CheZ generator with a fusion GFP reporter

This generator contains promoter J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains. Promoters with different strength are also constructed as BBa_K2012014[1],BBa_K2012016[2]

We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately
the image are as following


Figure 1.CL1 control was observed under light field and fluorescence field

.

Figure 2.E.coli with BBa_K2012013 was observed under light field and fluorescence field;

<img src="T--HZAU-China--CheZ-GFP_swarming.jpg" style="width:600px;margin-left:5%;"/>

Figure 3. The swarming plate of E.coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1362


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Categories
Parameters
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